Nematode Cancer Risk Test "N-NOSE®" Celebrates 10th Anniversary and Surpasses 1 Million Cumulative Samples

HIROTSU BIO SCIENCE Co., Ltd. (Headquarters: Chiyoda-ku, Tokyo; CEO: Takasuke Hirotsu; hereinafter referred to as "the Company") is pleased to announce that it has reached its 10th anniversary since the development of the nematode cancer risk test "N-NOSE®" and has surpassed a cumulative total of 1 million samples.

To commemorate this milestone, we will fully implement our proprietary AI image analysis technology, which utilizes the nematode's chemotaxis data accumulated from 1 million samples, into our testing process starting July 1, 2026.

Furthermore, to express our gratitude to all those who have used N-NOSE® and to provide an opportunity for more people to become interested in their health status, we will be holding the "N-NOSE® 10th Anniversary x 1 Million Samples Double Commemorative Campaign" starting on Wednesday, July 1, 2026.

Milestone of N-NOSE® 10th Anniversary and 1 Million Cumulative Samples

N-NOSE®, for which research and development began in 2016, has been developed with the aim of "making it easier for everyone to know their health status" by focusing on the superior olfactory capabilities of nematodes.

We interpret surpassing 1 million cumulative samples as a result of many people taking an interest in their health and choosing N-NOSE® as the first step towards healthy behavior.

Moreover, the data asset of 1 million samples serves as a crucial foundation to support future research and development and the advancement of our testing process.

Full Implementation of AI Analysis Utilizing Big Data from 1 Million Samples

Starting July 1, 2026, the Company will fully implement its proprietary AI image analysis technology, which utilizes the accumulated nematode chemotaxis data, into its testing process.

By leveraging AI technology, we aim to improve the efficiency of analyzing vast amounts of data and further optimize the testing process, thereby establishing a stable service provision system.

We will continue to advance our research and development towards realizing a "next-generation health infrastructure" that allows everyone to routinely check their health status by fusing biological capabilities with technology.

"N-NOSE® 10th Anniversary x 1 Million Samples Breakthrough Double Commemorative Campaign" Overview

● Period: Wednesday, July 1, 2026 – Wednesday, September 30, 2026

● Content: A "3,000 yen discount coupon" will be distributed, usable when purchasing an N-NOSE® test kit.

● Target Prices (including tax):

○ Standard Test Course: Normally \19,800 ⇒ \16,800

○ Regular Test Course: Normally \16,800 ⇒ \13,800 (※ First application only)

Comment from CEO Takasuke Hirotsu

"It has been 10 years since we started the development of N-NOSE®. Reaching the milestone of 1 million cumulative samples is thanks to the support of so many people. I am deeply grateful.

What we aim for is to make cancer testing not a special event, but an "everyday health infrastructure" that everyone can easily access.

We believe that the AI analysis we are launching now, utilizing the valuable data asset of 1 million samples, will become an important foundation supporting future service improvements and stable supply.

And through the campaign commemorating this milestone, we hope that not only those who have used our service so far, but also those who have not yet taken the test, will use it as an opportunity to face their own health.

We will continue to evolve without stopping, so that we can support the extension of your healthy lifespan in any era."

Reasons Why "N-NOSE®" Has Been Chosen by Many Over 10 Years

Since our establishment in 2016, the Company has aimed for a world where early cancer detection is commonplace. Despite cancer being the leading cause of death in Japan for many years, the screening participation rate has remained below 50%. This is due to psychological and physical hurdles associated with existing tests, such as "lack of time," "pain involved," and "high cost."

By utilizing the biological characteristics of nematodes, which have a superior sense of smell, N-NOSE® has made possible a "low-cost, simple, highly accurate, and comprehensive" primary screening test that was difficult to achieve with conventional artificial sensors. We have presented society with a new option: incorporating cancer risk testing into daily life through a non-invasive method of simply submitting a small amount of urine.

We believe that reaching the significant milestone of 1 million cumulative samples is a result of our efforts to "create an environment where anyone can easily manage their health" being accepted by many people.

About "N-NOSE®", the Nematode Cancer Test That Can Be Mailed

This is a cancer risk test that utilizes the mechanism by which nematodes (C. elegans), with their excellent sense of smell, detect the unique odors of cancer present in human urine. With the ability to transport samples at room temperature, it has become possible to more easily screen for cancer risk throughout the body simply by mailing urine samples in a postal box.

Service Site: https://lp.n-nose.com/

About HIROTSU BIO SCIENCE Co., Ltd.

We aim to "protect people's health and future peace of mind" through the research, development, and commercialization of unique testing technologies that leverage biological capabilities. Established in 2016, N-NOSE® was commercialized through the rich imagination and tireless efforts of our researchers. In the era of 100-year lifespans, while extending healthy life expectancy is emphasized, it is said that 1 in 2 people will develop cancer. We will contribute to solving these social issues with our groundbreaking, world-first technology that utilizes the superior olfactory power of nematodes, which surpasses artificial devices.

Location: 22nd Floor, New Otani Garden Court, 4-1 Kioicho, Chiyoda-ku, Tokyo

Representative: Takasuke Hirotsu, Representative Director and CEO

Establishment Date: August 2016

Main Business Activities: Research, development, and sales of cancer tests utilizing nematodes and nematode olfactory sensors

Corporate Site: https://hbio.jp/