Hydrogen Supplement Raw Material 'Hydroshell': Joint Research Confirms 15% Extension in Endurance Time When Combined with Exercise

ardesign Co., Ltd. (Headquarters: Minato-ku, Tokyo; Representative Director: Gota Kawano), a company that develops and supplies the hydrogen-generating supplement raw material 'Hydroshell,' announced the results of a study using its hydrogen-generating raw material 'Hydroshell' as a final report of joint research with Professor Chinobu Sakurai of the Graduate School of Sports Science, Toin University of Yokohama.

In this study, the group that orally ingested the hydrogen-generating supplement containing Hydroshell showed a further 15% extension in endurance time compared to the exercise-only group (Exercise-only EX group: 7.50 min → Hydrogen + Exercise H2+EX group: 8.60 min, p=0.043). Additionally, at the cellular and tissue levels, stepwise responses such as decreased oxidative stress markers, nuclear translocation of Nrf2, and a trend toward increased CS activity were observed.

Hydroshell is positioned not as a 'material that dramatically enhances endurance on its own,' but as a 'conditioning material focusing on metabolic response during exercise.' It can be used as basic research data for product development in the sports nutrition and conditioning fields.

[Three Research Results]
① +15% Extension in Endurance Time When Combined with Exercise (vs. Exercise Alone)
Time to exhaustion (average): Control group (CON) 5.73 min, Hydrogen only (H2) 6.40 min (vs. CON, p=0.054), Exercise only (EX) 7.50 min (vs. CON, p=0.043), Hydrogen + Exercise (H2+EX) 8.60 min (+15% vs. EX, p=0.043 / vs. CON, p<0.001). Combining hydrogen supplement intake with exercise showed a significant additional benefit compared to the exercise-only group.

② Observation of 'Three-Step Response' at the Cellular Level Over Time
1 hour after intake: Mitochondrial reactive oxygen species significantly decreased (tibialis anterior muscle: p=0.0037). 24 hours after intake: Nuclear Nrf2 significantly increased (fast-twitch muscle p=0.0157 / slow-twitch muscle p=0.0246). 7 days after intake: No significant change in ATP levels, while CS activity showed an increasing trend (p=0.0856).

③ Suggests Involvement in Metabolic Response During Exercise, Not Just 'Antioxidation'
This provides clues for understanding the action of oral hydrogen not merely as an antioxidant effect, but from the perspective of regulating the metabolic environment that supports exercise adaptation. Orally ingested hydrogen-generating materials may more gently influence biological responses through hydrogen generation in the digestive tract.

[Study Overview]
Subjects: Wistar male rats (9 weeks old). Administration: Oral administration of hydrogen-generating supplement 5mg/day. Evaluation time points: 1 hour / 24 hours / 7 days after intake. Exercise intervention: 4-group comparison study (CON / H2 / EX / H2+EX). Time to exhaustion on an animal treadmill was measured. Evaluation indicators: Mitochondrial reactive oxygen species (ROS), membrane potential, nuclear Nrf2, ATP levels, citrate synthase (CS) activity, endurance performance. Joint research partner: Professor Chinobu Sakurai, Graduate School of Sports Science, Toin University of Yokohama. Reporting period: February 2026 (final report).

[What is Hydroshell?]
Hydroshell is a hydrogen-generating supplement raw material designed based on the knowledge gained from supplying over 34 tons of hydrogen-generating related raw materials to domestic supplement and food manufacturers since the company's founding in 2013. In addition to university joint research data including this study, the company has prepared necessary materials for B2B adoption consideration, such as hydrogen generation tests by third-party organizations, quality tests, SDS, and raw material specifications. Evidence: Joint research with Toin University of Yokohama (+15% additional benefit under exercise combination conditions). Sustainability: Hydrogen generation confirmed by 24-hour cumulative measurement (GC-BID method, WHSA certified organization). Quality: Every lot is tested for arsenic, heavy metals, bacteria, coliforms, etc. at JFRL (Japan Food Research Laboratories). Test reports are issued upon delivery. Flexible supply: Supports small-lot supply from 1kg of raw material and 10 bags of PB packaged products. Also supports OEM, allowing smooth scaling from test sales to mass production. Compatible dosage forms: Hard capsules / Tablets / Stick powder.

[Expected Product Application Areas]
Sports nutrition (endurance, training combination, pre-workout), continuous intake supplements as daily conditioning materials, health maintenance and beauty conditioning, utilization of research data in health foods and supplements in general.

[Consultation for Raw Material Supply, PB Packaged Products, and OEM Manufacturing]
Ardesign offers 'Hydroshell' in the following three forms according to the customer's business scale and sales phase. ① Raw material supply (from 1kg): For supplement manufacturers who formulate and manufacture in-house. Complete with research materials, test reports, SDS, and raw material specifications. NDA execution possible. ② Ardesign PB packaged products (from 10 bags) currently available: For businesses without their own brand, ardesign supplies packaged products as finished goods in small lots from 10 bags. Suitable for immediate sales start, test sales, and novelty use for salons, clinics, EC, and subscription businesses. ③ OEM contract manufacturing: Provides integrated support from formulation design to packaging and advertising display support compliant with the Pharmaceutical and Medical Device Act. Extensive track record in formulation proposals for sports nutrition, conditioning, and beauty.

[Consultation Flow]
Inquiry (please inform us of your application, estimated lot size, and dosage form) → Free delivery of evaluation samples and research materials → Proposal of formulation design and quotation (including formulation design for OEM) → Manufacturing and delivery (test report attached).

[Contact Information]
Inquiry form: https://www.ardesign-h2.com/contact
Hydroshell details: https://www.ardesign-h2.com/hydroshellpowder

[Company Profile]
Company name: ardesign Co., Ltd.
Location: Hamamatsucho Dai, 2-2-15 Hamamatsucho, Minato-ku, Tokyo